Regulatory
Part:BBa_K2587002:Design
Designed by: Ylenia Longo Group: iGEM18_Duesseldorf (2018-09-15)
Plux
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 116
Design Notes
This sequence contains TypeII S restriction sites and is compatible with the use of the CIDAR toolbox, which is based on the Golden Gate cloning principle.
Source
This sequence was taken from GenBank and codon optimised for E. coli.